The Consumption of Beef on Appetite and Cognitive Function
Obesity, Poor Glycemic Control, Dietary Interventions

About this trial
This is an interventional prevention trial for Obesity focused on measuring High Protein, Satiety, Appetite Control, Protein Distribution, Cognitive Function, Ad libitum Food Intake, Obesity prevention, Obesity treatment
Eligibility Criteria
Inclusion Criteria:
- Women
- Ages: 18-52 y
- BMI: 25-32 kg/m2
- No metabolic, hormonal, and/or neural conditions/diseases that influence metabolism, appetite, or cognition
- No blood donations to American Red Cross within past 6 months
- No medication that would influence directly appetite or cognition
- No change in any medications (over the past 3 months)
- Non-smoking (for the past year)
- Not pregnant within the past 6 months (or planning to become pregnant during study)
- Have not given birth and/or lactating within the past 6 months
- Normal menstrual cycles between 26-32 days in duration; 5 menstrual cycles within the past 6 months; or able to provide documentation of oral/hormonal contraceptive use which contains low-dose estrogen/progesterone to maintain continuous levels throughout the 28-day cycle (i.e., no placebos)
- Not clinically diagnosed with an eating disorder
- No weight loss/gain (≥10 lb. in the past 6 months)
- No past history of surgical interventions for the treatment of obesity
- No allergies and/or aversions to the study foods, particularly beef
- Consumes ≤ 800 mg caffeine/day
- Of this, ≤260 mg caffeine is consumed prior to lunch (~12 oz. Starbucks coffee)
- Not currently and/or previously on a specific diet including high protein, vegan, vegetarian, etc.
- Willing and able to consume all study foods
- Habitually consumes breakfast, lunch, and dinner >4 days/week
- No history of drug abuse or alcohol abuse (i.e., >14 drinks/week; 1 drink=12 oz. beer; 5 oz. wine; 1.5 oz. liquor)
- Displays a score of <4 on the Three Factor Eating Habits Questionnaire (TFEQ)
- Conventional (typical) and consistent sleep patterns
- awake hours somewhere between 5 am - 11 pm with no afternoon naps
- rates quality of sleep as Fairly to Very Good on the Pittsburg Sleep Quality Index (PSQI)
- averages ≥ 6 sleep hours/night over the past month
- Displays a Profile of Mood State 2nd Edition (POMS2; 60-item) Depression-Dejection Scale score within 1.5 SD of the age, gender, and racial-specific normative mean (Nyenhuis et al., 1999; Petterson K et al., 2006)
- Obtained a "Yes" on the validity indicator and displayed a score of >70 (>2%) on the CNS Vital Signs Battery
- Sedentary (i.e., limited purposeful physical activity)
- Willing and able to maintain current inactivity patterns throughout the study
- Willing and able to follow all study procedures
- Generally healthy, as assessed from the medical history questionnaire
Exclusion Criteria:
- Men
- Ages: <18 or >52 y
- BMI: <25 or >32 kg/m2
- Any metabolic, hormonal, and/or neural conditions/diseases that influence metabolism, appetite, or cognition
- Have donated blood to American Red Cross within past 6 months
- Medication that would influence directly appetite or cognition
- Change in medications (over the past 3 months)
- Have smoked in the past year
- Pregnant within the past 6 months (or planning to become pregnant during study)
- Have given birth and/or lactating within the past 6 months
- Abnormal menstrual cycles [not between 26-32 days in duration; or not 5-6 menstrual cycles within the past 6 months; or not able to provide documentation of oral/hormonal contraceptive use which contains low-dose estrogen/progesterone to maintain continuous levels throughout the 28-day cycle (i.e., no placebos)]
- Clinically diagnosed with an eating disorder
- Weight loss/gain (≥10 lb. in the past 6 months)
- Past history of surgical interventions for the treatment of obesity
- Allergies and/or aversions to the study foods, particularly beef
- Consumes > 800 mg caffeine/day
- Or, of caffeine consumed, >260 mg caffeine is consumed prior to lunch (~12 oz. Starbucks coffee)
- Currently and/or previously on a specific diet including high protein, vegan, vegetarian, etc.
- Unwilling and/or unable to consume all study foods
- Habitually consumes breakfast, lunch, and dinner <4 days/week
- History of drug abuse or alcohol abuse (i.e., >14 drinks/week; 1 drink=12 oz. beer; 5 oz. wine; 1.5 oz. liquor)
- Displays a score of >4 on the Three Factor Eating Habits Questionnaire (TFEQ)
- Unconventional (atypical) and/or inconsistent sleep patterns
- awake hours not somewhere between 5 am - 11 pm and/or afternoon naps
- does not rate quality of sleep as Fairly to Very Good on the Pittsburg Sleep Quality Index (PSQI)
- averages < 6 sleep hours/night over the past month
- Does not display a Profile of Mood State 2nd Edition (POMS2; 60-item) Depression-Dejection Scale score within 1.5 SD of the age, gender, and racial-specific normative mean (Nyenhuis et al., 1999; Petterson K et al., 2006)
- Did not obtain a "Yes" on the validity indicator and/or did not display a score of >70 (>2%) on the CNS Vital Signs Battery
- Not sedentary (i.e., purposeful physical activity)
- Unwilling and/or unable to maintain current inactivity patterns throughout the study
- Unwilling and/or unable to follow all study procedures
- Not generally healthy, as assessed from the medical history questionnaire
Sites / Locations
Arms of the Study
Arm 1
Arm 2
Experimental
Experimental
Energy Balance Comparison
Energy Restriction Comparison
Participants will randomly consume 4 eucaloric diets for 7 consecutive days/treatment. Energy levels for all diets are established according to needs for energy balance. Interventions: Standard Protein-Plant, Even Distribution (SP-PLANT-EVEN); Standard Protein-Beef, Even Distribution (SP-BEEF-EVEN); High Protein-Beef, Even Distribution (HP-BEEF-EVEN); High Protein-Beef, Uneven Distribution (HP-BEEF-UNEVEN)
Participants will randomly consume 3 energy restriction diets (1250 kcal/day) for 7 consecutive days/treatment. Energy levels for all diets are established according to needs for energy restriction. Interventions: Standard Protein-Plant, Even Distribution (SP-PLANT-EVEN); Standard Protein-Beef, Even Distribution (SP-BEEF-EVEN); High Protein-Beef, Even Distribution (HP-BEEF-EVEN)